surface plasmon resonance based fiber optic sensor utilizing platinum layer Search Results


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Biacore spr experiments
(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface <t>plasmon</t> <t>resonance</t> <t>(SPR).</t> The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.
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Biacore surface plasmon resonance ship
(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface <t>plasmon</t> <t>resonance</t> <t>(SPR).</t> The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.
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Biacore instruments, methods and reagents
(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface <t>plasmon</t> <t>resonance</t> <t>(SPR).</t> The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.
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Texas Instruments optical biosensors based on surface plasmon resonance (spr) spectroscopy
(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface <t>plasmon</t> <t>resonance</t> <t>(SPR).</t> The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.
Optical Biosensors Based On Surface Plasmon Resonance (Spr) Spectroscopy, supplied by Texas Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface plasmon resonance (SPR). The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.

Journal: Science signaling

Article Title: Instructive roles for agonist binding parameters in determining the functional bandwidth of cytokine receptor signaling

doi: 10.1126/scisignal.aab2677

Figure Lengend Snippet: (A) Binding isotherms of the IL-13 agonists displayed on the surface of yeast. Yeast-displayed IL-13 agonists were incubated with the indicated concentrations of biotinylated IL-13Rα1 ECD, stained with Alexa-647-coupled Streptavidin, and assessed via flow cytometry. Sigmoidal curves were fitted using Prism software (GraphPad). (B) Normalized KD binding affinities of the recombinant IL-13 agonists determined via surface plasmon resonance (SPR). The IL-13 KD was fixed to one and other agonists were normalized accordingly. (C) Summary of the amino acids mutated in the IL-13 agonists as well as the kon, koff and KD binding parameters values obtained from SPR experiments.

Article Snippet: Surface plasmon resonance SPR experiments were conducted on a Biacore T100 instrument using a Biacore SA sensor chip (GE Healthcare).

Techniques: Binding Assay, Incubation, Staining, Flow Cytometry, Software, Recombinant, SPR Assay